Application of lanthanum stearate monolayers as a metal-affinity sorbent for the selective sorption of soman adducts to human serum albumin

V N Babakov; E V Shreiner; O A Keltsieva; Y A Dubrovskii; V V Shilovskikh; I M Zorin; N G Sukhodolov; I G Zenkevich; E P Podolskaya; A A Selyutin

doi:10.1016/j.talanta.2018.11.103

Application of lanthanum stearate monolayers as a metal-affinity sorbent for the selective sorption of soman adducts to human serum albumin

Talanta. 2019 Apr 1:195:728-731. doi: 10.1016/j.talanta.2018.11.103. Epub 2018 Dec 5.

Authors

Affiliations

¹ Research Institute of Hygiene, Occupational Pathology and Human Ecology, FMBA, 188663 St. Petersburg, Russia.
² Institute of Analytical Instrumentation RAS, 190103 St. Petersburg, Russia.
³ Institute of Analytical Instrumentation RAS, 190103 St. Petersburg, Russia; Institute of Toxicology FMBA, 192019 St. Petersburg, Russia.
⁴ Research Institute of Hygiene, Occupational Pathology and Human Ecology, FMBA, 188663 St. Petersburg, Russia; St. Petersburg State University, 199034 St. Petersburg, Russia.
⁵ St. Petersburg State University, 199034 St. Petersburg, Russia.
⁶ Institute of Analytical Instrumentation RAS, 190103 St. Petersburg, Russia; St. Petersburg State University, 199034 St. Petersburg, Russia.
⁷ St. Petersburg State University, 199034 St. Petersburg, Russia. Electronic address: a.selyutin@spbu.ru.

PMID: 30625608
DOI: 10.1016/j.talanta.2018.11.103

Abstract

A new metal-affinity sorbent based on lanthanum stearate monolayers has been developed and characterized. The prospect of its application to specific extraction of organophosphorous compound (OP) adducts of blood proteins was demonstrated. For this, the patterns of soman adducts of human serum albumin (HSA) were comprehensively characterized by matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS).

Keywords: Langmuir-Blodgett films; Lanthanum stearate; Organophosphorous compounds (OPs); Serum albumin adducts.

MeSH terms

Adsorption
Adult
Humans
Lanthanum / chemistry*
Male
Middle Aged
Serum Albumin, Human / chemistry*
Soman / chemistry*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Stearates / chemistry*

Substances

Stearates
Lanthanum
Soman
Serum Albumin, Human