Rapid detection of human mastadenovirus species B by recombinase polymerase amplification assay

BMC Microbiol. 2019 Jan 8;19(1):8. doi: 10.1186/s12866-018-1365-7.

Abstract

Background: As an important component of the causative agent of respiratory tract infections, enteric and eye infections, Human mastadenoviruses (HAdVs) species B spread easily in the crowd. In this study, we developed a recombinase polymerase amplification (RPA) assay for rapidly detecting HAdVs species B which was comprised of two different formats (real-time and lateral-flow device).

Results: This assay was confirmed to be able to detect 5 different HAdVs species B subtypes (HAdV-B3, HAdV-B7, HAdV-B11, HAdV-B14 and HAdV-B55) without cross-reactions with other subtypes and other respiratory tract pathogens. This RPA assay has not only highly sensitivity with low detection limit of 50 copies per reaction but also short reaction time (< 15 min per detection). Furthermore, the real-time RPA assay has excellent correlation with real-time PCR assay for detection of HAdVs species B presented in clinical samples.

Conclusions: Thus, the RPA assay developed in this study provides an effective and portable approach for the rapid detection of HAdVs species B.

Keywords: Human mastadenoviruses; Lateral-flow device; Real-time; Recombinase polymerase amplification.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA-Directed DNA Polymerase / metabolism*
  • Humans
  • Limit of Detection
  • Mastadenovirus / classification*
  • Mastadenovirus / genetics*
  • Molecular Typing / methods*
  • Nucleic Acid Amplification Techniques / standards*
  • Polymerase Chain Reaction / standards
  • Recombinases / metabolism*
  • Reproducibility of Results
  • Virology / methods*

Substances

  • Recombinases
  • DNA-Directed DNA Polymerase