Abstract
In this study, the antiobesity effects of n-butanol extract of lotus seeds (LBE) were evaluated in cultured 3T3-L1 preadipocytes and in high-fat diet (HFD)-fed mice. LBE decreased lipid contents in mature 3T3-L1 cells without obvious cytotoxicity. Meanwhile, LBE supplementation also led to weight loss and improved plasma lipid profiles in HFD-fed mice. Furthermore, LBE could activate AMP-activated protein kinase (AMPK) accompanied by down-regulation of lipogenesis related genes (PPARγ, aP2, LPL, C/EBPα, FAS, SREBP-1c) and up-regulation of lipolysis genes (adiponectin and PPARα) in vitro and in vivo. Collectively, our data demonstrated LBE possesses antiadipogenic and antilipogenic activities which are, at least partially, mediated by the activation of AMPK signaling pathways.
Keywords:
AMPK; adipogenesis; lipolysis; lotus seeds; n-butanol extract; obesity.
MeSH terms
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3T3-L1 Cells
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AMP-Activated Protein Kinases / genetics
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AMP-Activated Protein Kinases / metabolism*
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Adipocytes / drug effects*
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Adipocytes / enzymology
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Adipocytes / metabolism
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Adipogenesis / drug effects
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Animals
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Anti-Obesity Agents / administration & dosage*
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Anti-Obesity Agents / isolation & purification
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CCAAT-Enhancer-Binding Protein-alpha / genetics
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CCAAT-Enhancer-Binding Protein-alpha / metabolism
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Diet, High-Fat / adverse effects
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Humans
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Lipid Metabolism / drug effects
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Lipogenesis / drug effects
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Lotus / chemistry*
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Male
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Mice
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Mice, Inbred ICR
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Obesity / drug therapy*
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Obesity / genetics
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Obesity / metabolism
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Obesity / physiopathology
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PPAR gamma / genetics
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PPAR gamma / metabolism
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Plant Extracts / administration & dosage*
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Plant Extracts / isolation & purification
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Seeds / chemistry
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Sterol Regulatory Element Binding Protein 1 / genetics
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Sterol Regulatory Element Binding Protein 1 / metabolism
Substances
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Anti-Obesity Agents
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CCAAT-Enhancer-Binding Protein-alpha
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PPAR gamma
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Plant Extracts
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Sterol Regulatory Element Binding Protein 1
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AMP-Activated Protein Kinases