Though histochemical techniques have been used for decades, they are still very important in basic research. They make it possible to work on fixed tissues and provide a large amount of information in a relatively short time and at a low cost. Here we describe methods for indirect immunohistochemistry and immunofluorescence on sections of tadpoles and tissues of adult amphibians belonging to the species Xenopus laevis. The objective is to localize calpains within tissues in order to understand their involvement in cellular processes.
Keywords: Amphibians; Calpains; Embryonic development; Immunofluorescence; Immunohistochemistry.