Untargeted metabolite profiling and phytochemical analysis of Micromeria fruticosa L. (Lamiaceae) leaves

Ibrahim M Abu-Reidah; David Arráez-Román; Mohammed Al-Nuri; Ismail Warad; Antonio Segura-Carretero

doi:10.1016/j.foodchem.2018.11.144

Untargeted metabolite profiling and phytochemical analysis of Micromeria fruticosa L. (Lamiaceae) leaves

Food Chem. 2019 May 1:279:128-143. doi: 10.1016/j.foodchem.2018.11.144. Epub 2018 Dec 6.

Authors

Ibrahim M Abu-Reidah¹, David Arráez-Román², Mohammed Al-Nuri³, Ismail Warad³, Antonio Segura-Carretero⁴

Affiliations

¹ Analytical Chemistry Department, Faculty of Sciences, University of Granada, Avenida. Fuentenueva s/n, 18071 Granada, Spain; Research and Development Center of Functional Food (CIDAF), Health-Sciences Technological Park, Avenida del Conocimiento, 37, 18016 Granada, Spain; Industrial Chemistry Department, Faculty of Sciences, Arab American University, P.O. Box. 240, 13 Zababdeh-Jenin, Palestine. Electronic address: iabureidah@gmail.com.
² Analytical Chemistry Department, Faculty of Sciences, University of Granada, Avenida. Fuentenueva s/n, 18071 Granada, Spain; Research and Development Center of Functional Food (CIDAF), Health-Sciences Technological Park, Avenida del Conocimiento, 37, 18016 Granada, Spain. Electronic address: darraez@ugr.es.
³ Chemistry Department, Faculty of Science, An-Najah National University, P.O. Box. 7, 415 Nablus, Palestine.
⁴ Analytical Chemistry Department, Faculty of Sciences, University of Granada, Avenida. Fuentenueva s/n, 18071 Granada, Spain; Research and Development Center of Functional Food (CIDAF), Health-Sciences Technological Park, Avenida del Conocimiento, 37, 18016 Granada, Spain.

PMID: 30611472
DOI: 10.1016/j.foodchem.2018.11.144

Abstract

Micromeria fruticosa is an important crop, is widely used in the Mediterranean basin as food and in folk medicine, owing to its health-promoting properties, partially due to the secondary metabolite composition. However, complete information on the phyto-metabolites in M. fruticosa is still lacking. Plant leaves were extracted in methanol (80%), then the phyto-metabolites were separated on C18 column and an extensive characterization using UHPLC-DAD-ESI-QTOF-MS² method in two ionization modes was established. A total of 215 phenolics and other compounds were tentatively identified, offering the first comprehensive study available on the phytochemicals from M. fruticosa. Over 180 phytochemicals (87 flavonoids, 41 phenolic acids, 16 terpenoids, 8 sulfate derivatives, 7 iridoids, and others) are reported in Micromeria for the first time. M. fruticosa can be a promising source of functional ingredients and has use in the food, pharma and nutraceutical industries.

Keywords: Acacetin-7-O-rutinoside (PubChem CID: 5317025); Apigenin 7-O-rutinoside (PubChem CID: 9851181); Chlorogenic acid (PubChem CID: 1794427); Coumaroylagmatine (PubChem CID: 5280691); Flavonoids; Lamiaceae; Lithospermic acid (PubChem CID: 6441498); Micromeria fruticosa; Phenolics; Rosmarinic acid (PubChem CID: 5281792); Rutin (PubChem CID: 5280805); Sagerinic acid (PubChem CID: 23760102); Salvinorin C (PubChem CID: 11134539); Santaflavone (PubChem CID: 44258695); Secondary metabolites; Sulfate derivatives; Ultra-high-performance liquid chromatography-diode array detection-electrospray ionization-quadrupole time of flight-mass spectrometry (UHPLC-DAD-ESI-QTOF-MS(2)).

MeSH terms

Chromatography, High Pressure Liquid
Lamiaceae / chemistry*
Lamiaceae / metabolism
Phenols / analysis
Phenols / chemistry
Phytochemicals / analysis*
Phytochemicals / chemistry
Plant Extracts / chemistry
Plant Leaves / chemistry
Plant Leaves / metabolism
Spectrometry, Mass, Electrospray Ionization / methods

Substances

Phenols
Phytochemicals
Plant Extracts