MoO3-x nanodots with dual enzyme mimic activities as multifunctional modulators for amyloid assembly and neurotoxicity

Qiusen Han; Xinhuan Wang; Xueliang Liu; Yufei Zhang; Shuangfei Cai; Cui Qi; Chen Wang; Rong Yang

doi:10.1016/j.jcis.2018.12.093

MoO_3-x nanodots with dual enzyme mimic activities as multifunctional modulators for amyloid assembly and neurotoxicity

J Colloid Interface Sci. 2019 Mar 15:539:575-584. doi: 10.1016/j.jcis.2018.12.093. Epub 2018 Dec 27.

Authors

Qiusen Han¹, Xinhuan Wang², Xueliang Liu², Yufei Zhang², Shuangfei Cai², Cui Qi², Chen Wang³, Rong Yang⁴

Affiliations

¹ CAS Key Lab for Biomedical Effects of Nanomaterials and Nanosafety, Center of Materials Science and Optoelectronics Engineering, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, University of Chinese Academy of Sciences, Beijing 100190, PR China; Sino-Danish College, Sino-Danish Center for Education and Research, University of Chinese Academy of Sciences, Beijing 100190, PR China.
² CAS Key Lab for Biomedical Effects of Nanomaterials and Nanosafety, Center of Materials Science and Optoelectronics Engineering, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, University of Chinese Academy of Sciences, Beijing 100190, PR China.
³ CAS Key Lab for Biomedical Effects of Nanomaterials and Nanosafety, Center of Materials Science and Optoelectronics Engineering, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, University of Chinese Academy of Sciences, Beijing 100190, PR China; Sino-Danish College, Sino-Danish Center for Education and Research, University of Chinese Academy of Sciences, Beijing 100190, PR China. Electronic address: wangch@nanoctr.cn.
⁴ CAS Key Lab for Biomedical Effects of Nanomaterials and Nanosafety, Center of Materials Science and Optoelectronics Engineering, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, University of Chinese Academy of Sciences, Beijing 100190, PR China; Sino-Danish College, Sino-Danish Center for Education and Research, University of Chinese Academy of Sciences, Beijing 100190, PR China. Electronic address: yangr@nanoctr.cn.

PMID: 30611053
DOI: 10.1016/j.jcis.2018.12.093

Abstract

Development of effective inhibitors toward Aβ aggregation and reactive oxygen species (ROS) scavengers are of crucial therapeutic implications for Alzheimer's disease (AD). Herein, a novel agent with dual enzyme mimic activities has been fabricated as a multifunctional Aβ fibrillation modulator. MoO_3-x nanodots were synthesized by pulsed laser ablation (PLA) method in MoS₂ nanosheets solutions, which may act directly as numerous fine targets. MoO_3-x nanodots showed a uniform and monodispersed morphology, and the tiny dots were around 3-5 nm with a narrow size distribution. Due to the efficient charge transition between Mo⁵⁺/Mo⁶⁺ on the dots surface, MoO_3-x nanodots exhibited excellent catalase and SOD mimic activities, which were adopted to alleviate Aβ-mediated oxidative stress. Moreover, MoO_3-x nanodots can efficiently inhibit Aβ aggregation and destabilize the preformed fibrils, and eventually protect neuronal cells from apoptosis induced by Aβ. Taken together, MoO_3-x nanodots with multifunctional roles can act as a potential therapeutic strategy for treatment of amyloid induced neurotoxicity.

Keywords: Amyloid; Enzyme-mimic activity; MoO(3−x) nanodots; Neurotoxicity; Pulsed laser ablation.

MeSH terms

Amyloid beta-Peptides / chemistry*
Amyloid beta-Peptides / toxicity*
Apoptosis / drug effects
Catalase / chemistry
Cell Survival / drug effects
Humans
Molybdenum / chemistry*
Molybdenum / metabolism
Molybdenum / pharmacology*
Nanoparticles / chemistry*
Neurons / drug effects*
Neurons / metabolism
Neurons / pathology*
Oxidative Stress / drug effects
Oxides / chemistry*
Oxides / metabolism
Oxides / pharmacology*
Particle Size
Protein Aggregates / drug effects
Reactive Oxygen Species / analysis
Reactive Oxygen Species / metabolism
Superoxide Dismutase / chemistry
Surface Properties
Tumor Cells, Cultured

Substances

Amyloid beta-Peptides
Oxides
Protein Aggregates
Reactive Oxygen Species
molybdenum trioxide
Molybdenum
Catalase
Superoxide Dismutase