Correlative Light and Electron Microscopy of Autophagosomes

Methods Mol Biol. 2019:1880:199-209. doi: 10.1007/978-1-4939-8873-0_12.

Abstract

Live-cell imaging has been widely used to study autophagosome biogenesis and maturation. When combined with correlative electron microscopy, this approach can be extended to reveal ultrastructural details in three dimensions. The resolution of electron microscopy is needed when membrane contact sites and tubular connections between organelles are studied.

Keywords: Autophagosome; Electron tomography; Live-cell imaging; Phagophore; Serial sectioning.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Autophagosomes / ultrastructure*
  • Cell Survival
  • HeLa Cells
  • Humans
  • Image Processing, Computer-Assisted / methods
  • Microscopy, Confocal / methods*
  • Microscopy, Electron / instrumentation
  • Microscopy, Electron / methods*
  • Microtomy / methods
  • Microtubule-Associated Proteins / analysis
  • Optical Imaging / methods
  • Software

Substances

  • MAP1LC3A protein, human
  • Microtubule-Associated Proteins