Simultaneous nanostructure and chemical imaging of intact whole nematodes

Michael W M Jones; Nicholas W Phillips; Brian Abbey; Dominic J Hare; Grant A van Riessen; David J Vine; Martin D de Jonge; Gawain McColl

doi:10.1039/c8cc09664c

Simultaneous nanostructure and chemical imaging of intact whole nematodes

Chem Commun (Camb). 2019 Jan 25;55(8):1052-1055. doi: 10.1039/c8cc09664c. Epub 2019 Jan 4.

Authors

Michael W M Jones¹, Nicholas W Phillips², Brian Abbey³, Dominic J Hare⁴, Grant A van Riessen⁵, David J Vine⁶, Martin D de Jonge⁷, Gawain McColl⁴

Affiliations

¹ Central Analytical Research Facility, Institute of Future Environments, Queensland University of Technology, Brisbane, Queensland 4000, Australia. mw.jones@qut.edu.au and Australian Synchrotron, 800 Blackburn Road, Clayton, Victoria 3168, Australia and ARC Centre of Excellence in Advanced Molecular Imaging, La Trobe Institute for Molecular Sciences, La Trobe University, Victoria 3086, Australia.
² ARC Centre of Excellence in Advanced Molecular Imaging, La Trobe Institute for Molecular Sciences, La Trobe University, Victoria 3086, Australia and Department of Engineering Science, University of Oxford, Oxford, Oxfordshire OX1 3PJ, UK.
³ Department of Engineering Science, University of Oxford, Oxford, Oxfordshire OX1 3PJ, UK and Department of Chemistry and Physics, La Trobe Institute for Molecular Science, La Trobe University, Victoria 3086, Australia.
⁴ The Florey Institute of Neuroscience and Mental Health, The University of Melbourne, Parkville, Victoria, 3052, Australia.
⁵ Department of Chemistry and Physics, La Trobe Institute for Molecular Science, La Trobe University, Victoria 3086, Australia.
⁶ X-ray Science Division, Advanced Photon Source, Argonne National Laboratory, Argonne, IL 60439, USA.
⁷ Australian Synchrotron, 800 Blackburn Road, Clayton, Victoria 3168, Australia.

PMID: 30608504
DOI: 10.1039/c8cc09664c

Abstract

Biological X-ray fluorescence microscopy (XFM) is an important tool for determining quantitative distributions of bioinorganics and essential trace elements. Here we present a new analysis approach for rapid nanoscale ptychographic imaging and simultaneous chemical mapping of large radiation sensitive specimens without image degradation associated with probe evolution.