Synthesis and biophysical characteristics of riboflavin/HSA protein system on silver nanoparticles

M Voicescu; S Ionescu; V S Manoiu; M Anastasescu; O Craciunescu; L Moldovan

doi:10.1016/j.msec.2018.10.087

Synthesis and biophysical characteristics of riboflavin/HSA protein system on silver nanoparticles

Mater Sci Eng C Mater Biol Appl. 2019 Mar:96:30-40. doi: 10.1016/j.msec.2018.10.087. Epub 2018 Oct 30.

Authors

M Voicescu¹, S Ionescu², V S Manoiu³, M Anastasescu⁴, O Craciunescu³, L Moldovan³

Affiliations

¹ Romanian Academy, Institute of Physical Chemistry "Ilie Murgulescu", Splaiul Independentei 202, 060021 Bucharest, Romania. Electronic address: voicescu@icf.ro.
² Department of Physical Chemistry, University of Bucharest, Bd Regina Elisabeta 4-12, Bucharest 030018, Romania.
³ Department of Cellular and Molecular Biology, National Institute of R&D for Biological Sciences, Splaiul Independentei 296, 060031 Bucharest, Romania.
⁴ Romanian Academy, Institute of Physical Chemistry "Ilie Murgulescu", Splaiul Independentei 202, 060021 Bucharest, Romania.

PMID: 30606536
DOI: 10.1016/j.msec.2018.10.087

Abstract

Novel l‑Arginine‑Dextran70 based-silver nanoparticles (SNPs) (SNPs^Arg), functionalized with Riboflavin (RF) and Human Serum Albumin (HSA) were characterized by UV-Vis absorption, Transmission Electron Microscopy (TEM), Atomic Force Microscopy (AFM), fluorescence and circular dichroism spectroscopy, chemiluminescence and Neutral red assays. TEM analysis showed the formed faceted particles, large clumps/fused aggregates, nano-featured with the mean particle size of 41.60 nm. Chemiluminescence and Neutral red assays for in vitro antioxidant and cytotoxic activities of the SNPs^Arg/RF/HSA systems have been studied. It was pointed out that SNPs^Arg functionalized with RF and HSA resulted in a bio-nanosystem which leads to a high activity against oxygen free radicals, altered viability, morphology, apoptotic bodies and decreased cell density of L929 fibroblast cells. Results are relevant for understanding the redox properties of RF in the case of biological applications and especially for RF containing drugs.

Keywords: Oxidative stress; Proteins; Riboflavin; Silver nanoparticles; Spectroscopy.

MeSH terms

Animals
Arginine / chemistry*
Cell Line
Dextrans / chemistry*
Fibroblasts / cytology
Fibroblasts / metabolism*
Humans
Mice
Nanoparticles / chemistry*
Protein Biosynthesis / drug effects*
Riboflavin / pharmacology*
Serum Albumin, Human / pharmacology*
Silver / chemistry*

Substances

Dextrans
Silver
Arginine
Riboflavin
Serum Albumin, Human