Objective: To explore the effect of ambient fine particulate matters (PM(2.5)) exposure on nasal oxidative stress level in patients with allergic rhinitis (AR). Methods: A panel of sixty AR patients was recruited as subjects. Four repeated measurements were carried out from June 2017 to January 2018. Nasal irrigation solution was collected and examined for malondialdehyde (MDA) and superoxide dismutase (SOD). Monitoring data of atmospheric pollutants and meteorological factors during measurement periods were also collected. Time activity pattern combined with micro-environment monitoring method was used to evaluate PM(2.5) exposure. Mixed effect model was applied to analyze the relationship between PM(2.5) exposure concentration and oxidative stress level. Results: 49 subjects accomplished the four repeated measurements, and the mean±SD of their age was (36.7±8.4) years old. The median of MDA and SOD in four measurement periods was 3.70, 3.70, 5.58, 6.24 nmol/ml, and 105.50, 102.50, 95.00, 96.50 U/ml. The concentration of PM(2.5) exposure in four measurement periods was (40.0±2.7), (41.5±2.5), (52.3±5.9) and (74.7±4.9) μg/m(3), respectively. Results of single pollutant mixed effect model analysis showed that 0-, 1-, 2-, 3-day lag concentrations of PM(2.5) was significantly positively associated with MDA, about β (95%CI) estimated as 0.24 (0.17, 0.30), 0.34 (0.27, 0.41), 0.32 (0.20, 0.44) and 0.33 (0.23, 0.43), respectively. 0-, 1-, 2-day concentrations of PM(2.5) was significantly negatively associated with SOD, about β (95%CI) estimated as -0.99 (-1.66, -0.31), -1.35 (-2.08, -0.62) and -0.94 (-1.80, -0.07), respectively. Multivariate analysis found that lag 1-day concentration of PM(2.5) was still significantly associated with MDA and SOD after controlling for temperature, age and other influencing factors. For a 10 μg/m(3) increase of PM(2.5) concentration, MDA increased 0.26 (95%CI: 0.18, 0.33) nmol/ml, and SOD decreased 0.87 (95%CI: 0.21, 1.53) U/ml. Conclusion: Our results suggested that PM(2.5) exposure can aggravate the nasal oxidative stress response of AR patients.
目的： 探讨大气PM(2.5)暴露对过敏性鼻炎患者鼻腔氧化应激水平的影响。 方法： 采用定组设计，招募了60例居住在太原市城区的过敏性鼻炎患者，于2017年6月至2018年1月进行4次重复测量，采集鼻腔冲洗液并测量其中氧化应激指标丙二醛（MDA）和超氧化物歧化酶（SOD）；收集测量期间大气污染物和气象因素的监测数据，利用时间活动模式结合微环境监测法进行PM(2.5)暴露评估，采用混合效应模型分析PM(2.5)暴露浓度与氧化应激水平的关系。 结果： 共有49例患者完成了所有4次重复测量，年龄为（36.7±8.4）岁。4次测量研究对象MDA含量M分别为3.70、3.70、5.58和6.24 nmol/ml，SOD活力M分别为105.50、102.50、95.00和96.50 U/ml，PM(2.5)暴露水平分别为（40.0±2.7）、（41.5±2.5）、（52.3±5.9）和（74.7±4. 9）μg/m(3)。单污染物混合效应模型分析发现，MDA含量与滞后第0至3天（lag 0 d~lag 3 d）的PM(2.5)浓度均呈正相关，β（95%CI）值分别为0.24（0.17，0.30）、0.34（0.27，0.41）、0.32（0.20，0.44）、0.33（0.23，0.43）；SOD活力与lag 0 d~lag 2 d的PM(2.5)浓度均呈负相关，β（95%CI）值分别为-0.99（-1.66，-0.31）、-1.35（-2.08，-0.62）、-0.94（-1.80，-0.07）。多因素分析发现，控制气温、年龄等因素后，PM(2.5)浓度在lag 1 d与MDA含量和SOD活力存在关联，PM(2.5)浓度每升高10 μg/m(3)，MDA含量增加0.26（95%CI：0.18，0.33）nmol/ml，SOD活力下降0.87（95%CI：0.21，1.53）U/ml。 结论： PM(2.5)暴露可加重过敏性鼻炎患者鼻腔的氧化应激反应。.
Keywords: Anaphylaxis; Oxidative stress; PM(2).5; Particulate matter; Rhinitis.