CRISPR RNA-guided autonomous delivery of Cas9

Nat Struct Mol Biol. 2019 Jan;26(1):14-24. doi: 10.1038/s41594-018-0173-y. Epub 2018 Dec 31.

Abstract

Cas9 is an endonuclease that can be programed to autonomously deliver diverse effectors to specified genetic addresses. High-resolution structures of this protein and its associated CRISPR RNA guide explain the molecular mechanisms of CRISPR-RNA-guided DNA recognition and provide a molecular blueprint that has facilitated structure-guided functional remodeling. Here we retrace events that led from early efforts to understand the central role of Cas9 in CRISPR-mediated adaptive immunity to contemporary efforts aimed at developing and deploying this enzyme for programmable genetic editing.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Review

MeSH terms

  • Animals
  • CRISPR-Associated Protein 9 / genetics
  • CRISPR-Associated Protein 9 / metabolism
  • Clustered Regularly Interspaced Short Palindromic Repeats / genetics
  • Clustered Regularly Interspaced Short Palindromic Repeats / physiology*
  • Gene Editing / methods
  • Humans
  • RNA, Guide, CRISPR-Cas Systems / genetics
  • RNA, Guide, CRISPR-Cas Systems / metabolism*

Substances

  • RNA, Guide, CRISPR-Cas Systems
  • CRISPR-Associated Protein 9