Structure of the O-specific polysaccharide from a halophilic bacterium Halomonas ventosae RU5S2EL

Ibrahim M Ibrahim; Elena N Sigida; Maxim S Kokoulin; Yulia P Fedonenko; Svetlana A Konnova

doi:10.1016/j.carres.2018.12.010

Structure of the O-specific polysaccharide from a halophilic bacterium Halomonas ventosae RU5S2EL

Carbohydr Res. 2019 Feb 1:473:1-4. doi: 10.1016/j.carres.2018.12.010. Epub 2018 Dec 15.

Authors

Ibrahim M Ibrahim¹, Elena N Sigida², Maxim S Kokoulin³, Yulia P Fedonenko⁴, Svetlana A Konnova⁴

Affiliations

¹ N. G. Chernyshevsky Saratov State University, 83 Ulitsa Astrakhanskaya, Saratov, 410012, Russia; Department of Agricultural Microbiology, Faculty of Agriculture, Fayoum University, Fayoum, 63514, Egypt.
² Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 13 Prospekt Entuziastov, Saratov, 410049, Russia. Electronic address: si_elena@mail.ru.
³ G. B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of Russian Academy of Sciences, 159 Prospekt 100 Let Vladivostoku, Vladivostok 690022, Russia.
⁴ N. G. Chernyshevsky Saratov State University, 83 Ulitsa Astrakhanskaya, Saratov, 410012, Russia; Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 13 Prospekt Entuziastov, Saratov, 410049, Russia.

PMID: 30590154
DOI: 10.1016/j.carres.2018.12.010

Abstract

Halomonas ventosae RU5S2EL, a halophilic Gram-negative bacterium isolated from salt sediments of lake Elton (Russia), was cultivated and the lipopolysaccharide was extracted by the Westphal procedure. The O-specific polysaccharide (OPS) was obtained by mild acid hydrolysis of the lipopolysaccharide and was studied by sugar analysis along with ¹H and ¹³C NMR spectroscopy, including ¹H,¹H COSY, TOCSY, ROESY, ¹H,¹³C HSQC, and HMBC experiments as well as Smith degradation. The OPS was found to consist of branched pentasaccharide repeating units of the following structure.

Keywords: Halomonas ventosae; Halophilic bacterium; Lipopolysaccharide; O-specific polysaccharide; Structure.

MeSH terms

Carbohydrate Sequence
Halomonas / chemistry*
O Antigens / chemistry*

Substances

O Antigens