Occurrence of zoonotic Cryptosporidium and Giardia duodenalis species/genotypes in urban rodents

Tiong Kai Tan; Van Lun Low; Wern Hann Ng; Jamaiah Ibrahim; Daryi Wang; Chun Hoong Tan; Selvi Chellappan; Yvonne Ai Lian Lim

doi:10.1016/j.parint.2018.12.007

Occurrence of zoonotic Cryptosporidium and Giardia duodenalis species/genotypes in urban rodents

Parasitol Int. 2019 Apr:69:110-113. doi: 10.1016/j.parint.2018.12.007. Epub 2018 Dec 24.

Authors

Tiong Kai Tan¹, Van Lun Low², Wern Hann Ng³, Jamaiah Ibrahim⁴, Daryi Wang⁵, Chun Hoong Tan⁶, Selvi Chellappan⁶, Yvonne Ai Lian Lim⁷

Affiliations

¹ Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia; Biodiversity Research Center, Academia Sinica, Taipei, Taiwan. Electronic address: stanley.monas@gmail.com.
² Tropical Infectious Diseases Research and Education Centre (TIDREC), University of Malaya, Kuala Lumpur, Malaysia.
³ Department of Bioscience and Sport Science, Faculty of Applied Science and Computing, Tunku Abdul Rahman University College, Kuala Lumpur, Malaysia; School of Biomolecular and Biomedical Science, Conway Institute, University College Dublin, Belfield, Dublin, Ireland.
⁴ Faculty of Medicine and Defence Health, National Defence University of Malaysia, Malaysia.
⁵ Biodiversity Research Center, Academia Sinica, Taipei, Taiwan.
⁶ Department of Bioscience and Sport Science, Faculty of Applied Science and Computing, Tunku Abdul Rahman University College, Kuala Lumpur, Malaysia.
⁷ Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia. Electronic address: limailian@um.edu.my.

PMID: 30590124
DOI: 10.1016/j.parint.2018.12.007

Abstract

This report describes the detection of zoonotic Cryptosporidium muris, C. parvum subgenotype IIa and Giardia duodenalis genotype B in urban rodents in Malaysia. A rare occurrence of C. meleagridis was also reported suggesting a role of rodents in mechanical transmission of this pathogen. Utilization of DNA sequencing and subtyping analysis confirmed the presence of zoonotic C. parvum subtypes IIaA17G2R1 and IIaA16G3R1 for the first time in rodents.

Keywords: 18S rRNA; C. muris; C. parvum; Cryptosporidium meleagridis; Giardia; Malaysia; gp60; tpi.

MeSH terms

Animals
Cryptosporidiosis / epidemiology*
Cryptosporidiosis / transmission
Cryptosporidium / genetics*
DNA, Protozoan / genetics
Feces / parasitology
Genotype*
Giardia lamblia / genetics*
Giardiasis / epidemiology
Giardiasis / transmission
Giardiasis / veterinary*
Malaysia / epidemiology
Phylogeny
RNA, Ribosomal, 18S / genetics
Rodentia / parasitology*
Sequence Analysis, DNA
Urban Renewal
Zoonoses / epidemiology
Zoonoses / parasitology

Substances

DNA, Protozoan
RNA, Ribosomal, 18S