Mapping of crown rust resistance gene Pc53 in oat (Avena sativa)

PLoS One. 2018 Dec 26;13(12):e0209105. doi: 10.1371/journal.pone.0209105. eCollection 2018.

Abstract

Crown rust disease caused by the fungus Puccinia coronata f. sp. avenae (Pca) is a major production constraint of oat in North America, Europe, and Australia. There are over 100 genes effective against one or more Pca races, but only a handful of seedling resistance (Pc) genes have been mapped to a known chromosomal location. The goal of the present study was to use linkage mapping to identify the genomic location of the Pc53 gene, and to produce a list of linked SNPs with potential as molecular markers for marker assisted breeding. The Pc53 gene was placed on the linkage group Mrg08 at 82.4 cM using F5-derived recombinant inbred lines (RILs) from a cross between the Pc53 carrier 6-112-1-15 (PI 311624) and the susceptible cultivar Otana. The map location was validated using RILs from a cross between 6-112-1-15 and the Pc50 differential line. Single nucleotide polymorphism marker GMI_ES02_c14533_567 was the closest to Pc53. A major seedling resistance gene 'PcKM' and QTL QcC.Core.08.1, QCr.Core.08.2, QCr.Core.08.3 and QCr.cdl9-12D were previously reported on Mrg08. QPc.Core.08.1 and PcKM were mapped to within 1 cM of Pc53; but previous virulence studies have indicated separate identities. The chromosomal location of Pc53 and SNPs linked with it will facilitate the utilization of Pc53 in oat breeding programs.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Avena / genetics*
  • Avena / growth & development
  • Chromosome Mapping
  • Disease Resistance / genetics*
  • Genetic Linkage
  • Genome, Plant*
  • Genotype
  • Phenotype
  • Plant Diseases / genetics
  • Plant Proteins / genetics*
  • Polymorphism, Single Nucleotide
  • Quantitative Trait Loci
  • Seedlings / genetics

Substances

  • Plant Proteins

Grants and funding

This research was supported in part by North American Millers' Association 58-2050-5-010 to KEK, and by an appointment of the first author to the Agricultural Research Service (ARS) Research Participation Program administered by the Oak Ridge Institute for Science and Education (ORISE) through an interagency agreement between the U.S. Department of Energy (DOE) and the U.S. Department of Agriculture (USDA). ORISE is managed by ORAU under DOE contract number DE-SC0014664. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.