Urinary Cell-Free DNA: Isolation, Quantification, and Quality Assessment

Methods Mol Biol. 2019:1909:211-221. doi: 10.1007/978-1-4939-8973-7_16.

Abstract

Urine cell-free DNA is an important source of diagnostic markers for different diseases (e.g., cancer and prenatal diagnosis). It is important to achieve a simple and fast protocol to maximize the recovery of DNA from urine supernatant and to assess its quality. Here we describe a simple approach from urine collection to DNA quality assessment for downstream analyses.

Keywords: Cell-free DNA; Isolation; Protocol; Quality; Quantification; Urine.

MeSH terms

  • Cell-Free Nucleic Acids / isolation & purification
  • Cell-Free Nucleic Acids / urine*
  • Female
  • Fluorometry / methods*
  • Humans
  • Neoplasms / diagnosis
  • Neoplasms / urine
  • Pregnancy
  • Prenatal Diagnosis / methods
  • Quality Control
  • Urinalysis / methods
  • Urine Specimen Collection / methods*

Substances

  • Cell-Free Nucleic Acids