Formation of nanoscale Te0 and its effect on TeO32- reduction in CH4-based membrane biofilm reactor

Abstract

Formation and recovery of elemental tellurium (Te⁰) from wastewaters are required by increasing demands and scarce resources. Membrane biofilm reactor (MBfR) using gaseous electron donor has been reported as a low-cost and benign technique to reduce and recover metal (loids). In this study, we demonstrate the feasibility of nanoscale Te⁰ formation by tellurite (TeO₃^2-) reduction in a CH₄-based MBfR. Biogenic Te⁰ intensively attached on cell surface, within diameters ranging from 10 nm to 30 nm and the hexagonal nanostructure. Along with the Te⁰ formation, the TeO₃^2- reduction was inhibited. After flushing, biofilm resumed the TeO₃^2- reduction ability, suggesting that the formed nanoscale Te⁰ might inhibit the reduction by hindering substrate transfer of TeO₃^2- to microbes. The 16S rRNA gene amplicon sequencing revealed that Thermomonas and Hyphomicrobium were possibly responsible for TeO₃^2- reduction since they increased consecutively along with the experiment operation. The PICRUSt (Phylogenetic Investigation of Communities by Reconstruction of Unobserved States) analysis showed that the sulfite reductases were positively correlated with the TeO₃^2- flux, indicating they were potential enzymes involved in reduction process. This study confirms the capability of CH₄-based MBfR in tellurium reduction and formation, and provides more techniques for resources recovery and recycles.

Keywords: Membrane biofilm reactor; Sulfite reductase; Tellurite reduction; Tellurium formation; Temporary inhibition.