Biodegradation of pesticide pollution is often restricted by environmental pressures, such as nutrient deprivation. Ensifer adhaerens CGMCC 6315 could overcome this issue and degrade neonicotinoid acetamiprid (ACE) efficiently under low nutrient stimuli. The ACE degradation rate improved by 33.1-fold when the lysogeny broth content for cell culture was decreased to 1/15-fold. Resting cells of CGMCC 6315 degraded 94.4% of 200 mg/L ACE in 12 h and quickly eliminated 87.8% of 5 mg/kg of residual soil ACE within 2 d. ACE degradation by CGMCC 6315 was via a nitrile hydratase (NHase) pathway. Genome sequencing showed that CGMCC 6315 had two NHase genes ( cnhA and pnhA). PnhA had the highest reported activity of 28.8 U/mg for ACE. QPCR and proteomic analysis showed that the improved ACE degradation ability was attributed to the up-regulated expression of PnhA. This biodegradation system of CGMCC 6315 has great potential for use in pesticide pollution remediation.
Keywords: Ensifer adhaerens; acetamiprid; biodegradation; nitrile hydratase; nutrient deprivation.