Expression, purification, crystallization and preliminary X-ray diffraction analysis of swine leukocyte antigen 2 complexed with a CTL epitope AS64 derived from Asia1 serotype of foot-and-mouth disease virus

BMC Vet Res. 2018 Dec 18;14(1):407. doi: 10.1186/s12917-018-1742-3.

Abstract

Background: Currently, the structural characteristics of the swine major histocompatibility complex (MHC) class I molecule, also named swine leukocyte antigen class I (SLA-I) molecule need to be further clarified.

Results: A complex of SLA-I constituted by an SLA-2*HB01 molecule with swine β2-microglobulin and a cytotoxic T lymphocyte (CTL) epitope FMDV-AS64 (ALLRSATYY) derived from VP1 protein (residues 64-72) of Asia 1 serotype of foot-and-mouth disease virus (FMDV) was expressed, refolded, purified and crystallized. By preliminary X-ray diffraction analysis, it was shown that the diffraction resolution of the crystal was 2.4 Å and the space group belonged to P212121 with unit cell parameters a = 48.37, b = 97.75, c = 166.163 Å.

Conclusion: This research will be in favor of illuminating the structural characteristics of an SLA-2 molecule associated with a CTL epitope derived from Asia1 serotype of FMDV.

Keywords: Asia 1 serotype; Crystal structure; Foot-and-mouth disease virus; Swine leukocyte antigen 2.

MeSH terms

  • Animals
  • Crystallization
  • Epitopes* / chemistry
  • Epitopes* / genetics
  • Epitopes* / isolation & purification
  • Epitopes* / metabolism
  • Foot-and-Mouth Disease Virus / genetics*
  • Histocompatibility Antigens Class I* / chemistry
  • Histocompatibility Antigens Class I* / genetics
  • Histocompatibility Antigens Class I* / isolation & purification
  • Histocompatibility Antigens Class I* / metabolism
  • Models, Molecular*
  • Protein Folding
  • Protein Structure, Quaternary
  • Serogroup
  • Swine
  • T-Lymphocytes, Cytotoxic / metabolism
  • X-Ray Diffraction*

Substances

  • Epitopes
  • Histocompatibility Antigens Class I
  • swine leukocyte antigen