DNA Aptamers in the Detection of Leukemia Cells by the Thickness Shear Mode Acoustics Method

Chemphyschem. 2019 Feb 18;20(4):545-554. doi: 10.1002/cphc.201801126. Epub 2019 Feb 6.

Abstract

By using the thickness shear mode acoustics method (TSM) and single-molecule force spectroscopy (SMFS) we studied the interactions between DNA aptamers (sgc8c) specific to the protein tyrosine kinase 7 (PTK7), which is localized in the membranes of leukemia lymphoblastics (MOLT-4), and lymphocyte (Jurkat) cell lines, as well with PTK7-negative U266 myeloid leukemia cells. The TSM method allowed the development of a highly sensitive, label-free biosensor for the detection leukemia cells with a limit of detection of (195±20) cells/mL. SMFS approved the high selectivity of the sgc8c aptamers to the PTK7 receptors at the cell surface and allowed determining the binding probability of the aptamers to the PTK7 receptors at different cell lines.

Keywords: DNA aptamers; PTK7; atomic force microscopy; leukemia; thickness shear mode.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acoustics
  • Aptamers, Nucleotide / chemistry*
  • Biosensing Techniques / methods*
  • Cell Adhesion Molecules / chemistry
  • Cell Line, Tumor
  • DNA / chemistry*
  • Humans
  • Leukemia / blood
  • Leukemia / diagnosis*
  • Limit of Detection
  • Receptor Protein-Tyrosine Kinases / chemistry

Substances

  • Aptamers, Nucleotide
  • Cell Adhesion Molecules
  • DNA
  • PTK7 protein, human
  • Receptor Protein-Tyrosine Kinases