Syngeneic, in contrast to allogeneic, mesenchymal stem cells have superior therapeutic potential following spinal cord injury

Ramil Hakim; Ruxandra Covacu; Vasilios Zachariadis; Arvid Frostell; Sreenivasa Sankavaram; Mikael Svensson; Lou Brundin

doi:10.1016/j.jneuroim.2018.11.005

Syngeneic, in contrast to allogeneic, mesenchymal stem cells have superior therapeutic potential following spinal cord injury

J Neuroimmunol. 2019 Mar 15:328:5-19. doi: 10.1016/j.jneuroim.2018.11.005. Epub 2018 Nov 16.

Authors

Ramil Hakim¹, Ruxandra Covacu², Vasilios Zachariadis³, Arvid Frostell⁴, Sreenivasa Sankavaram⁵, Mikael Svensson⁶, Lou Brundin⁷

Affiliations

¹ Center for Molecular Medicine, Karolinska Institutet, Solna 17176, Stockholm, Sweden; Department of Clinical Neuroscience, Karolinska Institutet, Solna 17176, Stockholm, Sweden. Electronic address: ramil.hakim@ki.se.
² Center for Molecular Medicine, Karolinska Institutet, Solna 17176, Stockholm, Sweden; Department of Clinical Neuroscience, Karolinska Institutet, Solna 17176, Stockholm, Sweden. Electronic address: ruxandra.covacu@ki.se.
³ Department of Oncology and Pathology, Karolinska Institutet, Solna 17176, Stockholm, Sweden. Electronic address: vasilios.zachariadis@ki.se.
⁴ Department of Clinical Neuroscience, Karolinska Institutet, Solna 17176, Stockholm, Sweden. Electronic address: arvid.frostell@ki.se.
⁵ Center for Molecular Medicine, Karolinska Institutet, Solna 17176, Stockholm, Sweden; Department of Clinical Neuroscience, Karolinska Institutet, Solna 17176, Stockholm, Sweden. Electronic address: sreenivasa.sankavaram@ki.se.
⁶ Department of Neurology and Neurosurgery, Karolinska University Hospital, BioClinicum, Solnavägen 30, Solna, Stockholm 17176, Sweden; Department of Clinical Neuroscience, Karolinska Institutet, Solna 17176, Stockholm, Sweden. Electronic address: mikael.svensson@ki.se.
⁷ Department of Neurology and Neurosurgery, Karolinska University Hospital, BioClinicum, Solnavägen 30, Solna, Stockholm 17176, Sweden; Center for Molecular Medicine, Karolinska Institutet, Solna 17176, Stockholm, Sweden; Department of Clinical Neuroscience, Karolinska Institutet, Solna 17176, Stockholm, Sweden. Electronic address: lou.brundin@ki.se.

PMID: 30551037
DOI: 10.1016/j.jneuroim.2018.11.005

Abstract

We evaluated the importance of histocompatibility of transplanted MSCs in terms of therapeutic potential. Mouse syngeneic MSCs or allogeneic MSCs were transplanted following SCI in mouse. In this study we found that syngeneic, but not allogeneic, MSCs alternatively activated macrophages resulting in a down-regulation of pro-inflammation. Syngeneic MSCs also had a general suppressive effect on the immune response as compared to allogeneic MSCs. Additionally, syngeneic, but not allogeneic, MSCs significantly enhanced the recovery of hind limb function. In this study we show that the histocompatibility of transplanted MSCs is of importance for their therapeutic potential.

Keywords: Graft survival; Hind limb function; Histocompatibility; Immune response; Mesenchymal stem cell; Neuroinflammation; Spinal cord injury.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Mesenchymal Stem Cell Transplantation / methods*
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Recovery of Function
Spinal Cord Injuries / immunology
Spinal Cord Injuries / therapy*
Transplantation, Homologous / methods*
Transplantation, Isogeneic / methods*