Abstract
The bark of Ruyschiaphylladenia was collected from Monteverde, Costa Rica, and extracted with acetone. Bioactivity-directed chromatographic separation of the crude acetone bark extract of R. phylladenia led to isolation and identification of lupeol, betulinic acid, and isofraxidin. Lupeol and betulinic acid showed in-vitro cytotoxic activity to MCF-7, MDA-MB-231, and 5637 human tumor cell lines. Isofraxidin was not cytotoxic, but did show antileishmanial activity to Leishmania amazonensis promastigotes.
MeSH terms
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Antineoplastic Agents, Phytogenic / chemistry
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Antineoplastic Agents, Phytogenic / pharmacology*
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Antiparasitic Agents / chemistry
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Antiparasitic Agents / pharmacology*
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Betulinic Acid
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Cell Line, Tumor
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Costa Rica
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Coumarins / chemistry
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Coumarins / pharmacology
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Drug Screening Assays, Antitumor
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Ericales / chemistry*
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Humans
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Leishmania / drug effects*
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Pentacyclic Triterpenes / analysis
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Pentacyclic Triterpenes / chemistry
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Plant Bark / chemistry*
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Plant Extracts / chemistry
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Plant Extracts / pharmacology*
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Triterpenes / chemistry
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Triterpenes / pharmacology
Substances
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Antineoplastic Agents, Phytogenic
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Antiparasitic Agents
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Coumarins
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Pentacyclic Triterpenes
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Plant Extracts
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Triterpenes
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isofraxidin
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lupeol
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Betulinic Acid