Data on hypoxia-induced VEGF, leptin and NF-kB p65 expression

Azizah Al-Anazi; Ranjit Parhar; Soad Saleh; Reem Al-Hijailan; Angela Inglis; Mansour Al-Jufan; Mohammed Bazzi; Sarwar Hashmi; Walter Conca; Kate Collison; Futwan Al-Mohanna

doi:10.1016/j.dib.2018.10.147

Data on hypoxia-induced VEGF, leptin and NF-kB p65 expression

Data Brief. 2018 Nov 1:21:2395-2397. doi: 10.1016/j.dib.2018.10.147. eCollection 2018 Dec.

Authors

Azizah Al-Anazi¹, Ranjit Parhar¹, Soad Saleh¹, Reem Al-Hijailan¹, Angela Inglis¹, Mansour Al-Jufan², Mohammed Bazzi³, Sarwar Hashmi⁴, Walter Conca^{1

5

6}, Kate Collison¹, Futwan Al-Mohanna^{1

6}

Affiliations

¹ Department of Cell Biology, Riyadh 11211, Saudi Arabia.
² Heart Centre, King Faisal Specialist Hospital and Research Centre, Riyadh 11211, Saudi Arabia.
³ Department of Biochemistry, College of Science, King Saud University, Riyadh 12372, Saudi Arabia.
⁴ Developmental Biology, Center for Vector Biology, Rutgers University, New Brunswick, NJ 08901, United States.
⁵ Department of Medicine, King Faisal Specialist Hospital and Research Centre, Alfaisal University, Riyadh 11211, Saudi Arabia.
⁶ College of Medicine, Alfaisal University, Riyadh 11211, Saudi Arabia.

Abstract

The data set presented here is associated with the article "Intracellular calcium and NF-_kB regulate hypoxia-induced leptin, VEGF, IL-6 and adiponectin secretion in human adipocytes" (Al-Anazi et al., 2018). Data illustrate hypoxia-induced VEGF and leptin expression in human adipocytes treated with the calcium chelator BAPTA-AM (1 µM). It also shows NF-κB p65 induced expression by hypoxia. Preadipocytes were differentiated for 14 days and then subjected to 0.5-1.5% oxygen in the presence and absence of BAPTA-AM or the NF-κB inhibitor SN50 for 48 h prior to RNA isolation and PCR analysis.