Determination of Rheumatoid Factors by ELISA

Nicole Hartwig Trier; Gunnar Houen

doi:10.1007/978-1-4939-8949-2_23

Determination of Rheumatoid Factors by ELISA

Methods Mol Biol. 2019:1901:263-270. doi: 10.1007/978-1-4939-8949-2_23.

Authors

Nicole Hartwig Trier¹, Gunnar Houen^{2

3}

Affiliations

¹ Department of Autoimmunology, Statens Serum Institut, Copenhagen, Denmark. nhp@ssi.dk.
² Department of Autoimmunology, Statens Serum Institut, Copenhagen, Denmark.
³ Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense, Denmark.

PMID: 30539586
DOI: 10.1007/978-1-4939-8949-2_23

Abstract

IgM and IgA autoantibodies binding to IgG are called rheumatoid factors (RFs) and occur with high frequency in rheumatoid arthritis (RA) and with lower frequency in other autoimmune diseases. RFs have diagnostic and prognostic value in RA, but they also have a high potential to cause false positive reactions in other immunoassays, especially sandwich assays. For these reasons it is imperative to be able to measure RFs in serum samples from patients suspected of RA or other autoimmune diseases and in serum samples to be analyzed by sandwich immunoassay for various clinical parameters. Here, a simple ELISA for IgM and IgA RFs is described.

Keywords: Autoantibodies; ELISA; Immunoassay; Rheumatoid arthritis; Rheumatoid factors; Sandwich immunoassay.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Enzyme-Linked Immunosorbent Assay / methods*
Humans
Immunoassay
Immunoglobulin M / metabolism
Reference Standards
Rheumatoid Factor / analysis*

Substances

Immunoglobulin M
Rheumatoid Factor