Comparison of vitamin D metabolites in wild and captive baboons

Toni E Ziegler; Amita Kapoor; Neil C Binkley; Karen S Rice; Jeffrey Rogers; Clifford J Jolly; Jane E Phillips-Conroy

doi:10.1002/ajp.22935

Comparison of vitamin D metabolites in wild and captive baboons

Am J Primatol. 2018 Dec;80(12):e22935. doi: 10.1002/ajp.22935.

Authors

Toni E Ziegler^{1

2}, Amita Kapoor^{1

2}, Neil C Binkley^{2

3}, Karen S Rice⁴, Jeffrey Rogers^{1

5}, Clifford J Jolly⁶, Jane E Phillips-Conroy^{7

8}

Affiliations

¹ Wisconsin National Primate Research Center, University of Wisconsin-Madison, Madison, Wisconsin.
² Institute of Clinical and Translational Research, University of Wisconsin-Madison, Madison, Wisconsin.
³ Department of Medicine, University of Wisconsin- Madison, Madison, Wisconsin.
⁴ Southwest National Primate Research Center, Texas Biomedical Research Institute, San Antonio, Texas.
⁵ Human Genome Sequencing Center, Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas.
⁶ Department of Anthropology, New York University, New York, New York.
⁷ Department of Neuroscience, Washington University School of Medicine, Saint Louis, Missouri.
⁸ Department of Anthropology, Department of Neuroscience, Washington University, Saint Louis, Missouri.

Abstract

Vitamin D adequacy is essential for multiple physiologic processes. With limited exposure to sunlight for vitamin D₃ synthesis, captive primates are supplemented with vitamin D₃ (cholecalciferol). Vitamin D metabolite data from wild primates living indigenously could suggest optimum levels. The purpose of this study was to: 1) to explore whether baboons, a speciose genus whose members have significant exposed skin, coat color variation and wide geographical distribution, mirrors the skin pigmentation-vitamin D relationship found in humans; 2) compare vitamin D metabolite levels in wild and captive members of the same or similar baboon species; and 3) apply a recently developed method currently used in humans for measuring multiple vitamin D metabolites as a panel to explore if/how these metabolites can inform us on vitamin D sufficiency. Serum samples from males of three baboon species in the wild: Papio anubis (olive baboon, dark exposed skin), P. cynocephalus (yellow baboon, brown exposed skin), and P. hamadryas (hamadryas baboon, pink exposed skin), were compared with vitamin D supplemented captive olive baboons with sun exposure. Liquid chromatography/tandem mass spectrometry (LC/MS/MS) measured vitamin D and its main metabolites. Cholecalciferol, 25 hydroxyvitamin D_2&3 (25(OH)D_2&3 ), and 24,25 dihydroxyvitamin D_2&3 (24,25(OH)₂ D_2&3 ), showed significant differences by species. The levels of cholecalciferol due to supplements in the captive olive baboons did not convert to higher 25(OH)D₃ while the wild olive baboons exhibited the lowest levels for both cholecalciferol and 25(OH)D₃ . Further metabolic conversion of 25(OH)D₃ to 24,25(OH)₂ D₃ indicated that all baboons had more similar conversion ratios and these were within the same range found for humans that are depicted as having adequate vitamin D levels. This study provided evidence that exposed skin color does influence vitamin D3 levels, with lower levels in darker skinned species, but these differences are eliminated in the downstream metabolite conversion indicating strong regulatory control.

Keywords: baboons; captive; metabolic profiles; skin color; vitamin D; wild.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Africa South of the Sahara
Aging
Animal Distribution
Animals
Animals, Wild*
Animals, Zoo*
Dietary Supplements
Male
Papio / blood*
Papio / metabolism
Skin Pigmentation
Species Specificity
Vitamin D / administration & dosage
Vitamin D / blood
Vitamin D / metabolism
Vitamin D / pharmacology*
Vitamin D Deficiency / prevention & control

Substances

Vitamin D

Abstract

Publication types

MeSH terms

Substances

Grants and funding