Mitochondrial Isolation and Real-Time Monitoring of MOMP

Methods Mol Biol. 2019:1877:121-130. doi: 10.1007/978-1-4939-8861-7_8.

Abstract

Isolated model systems have proven to be the standard in the apoptosis field to deconstruct MOMP into individual steps and to study the behavior of a subset of MOMP regulators. Here we describe the method to isolate, JC-1-label, and purify mouse liver mitochondria and subsequently describe how to utilize the JC-1-labeled mitochondria for real-time MOMP measurements.

Keywords: BAX; BCL-2 family; Isolated mitochondria; JC-1 labeling; Real-time MOMP measurements.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis / physiology
  • Female
  • Mice
  • Mice, Inbred C57BL
  • Mitochondria, Liver / metabolism*
  • Mitochondrial Membranes / metabolism*
  • bcl-2-Associated X Protein / metabolism

Substances

  • bcl-2-Associated X Protein