Complete Genome Sequences of Bordetella pertussis Clinical Isolate FR5810 and Reference Strain Tohama from Combined Oxford Nanopore and Illumina Sequencing

Valérie Bouchez; Sarah Louise Baines; Sophie Guillot; Sylvain Brisse

doi:10.1128/MRA.01207-18

Complete Genome Sequences of Bordetella pertussis Clinical Isolate FR5810 and Reference Strain Tohama from Combined Oxford Nanopore and Illumina Sequencing

Microbiol Resour Announc. 2018 Nov 15;7(19):e01207-18. doi: 10.1128/MRA.01207-18. eCollection 2018 Nov.

Authors

Valérie Bouchez^{1

2}, Sarah Louise Baines³, Sophie Guillot^{1

2}, Sylvain Brisse^{1

2}

Affiliations

¹ Biodiversity and Epidemiology of Bacterial Pathogens Unit, Institut Pasteur, Paris, France.
² National Reference Center for Whooping Cough and Other Bordetella Infections, Institut Pasteur, Paris, France.
³ Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Australia.

Abstract

Here, we describe the complete genome sequences of two Bordetella pertussis strains, FR5810, a clinical isolate recovered from the respiratory tract of an infant, and Tohama, a key reference strain for the species. Sequences were obtained using a hybrid approach combining Oxford Nanopore Technologies MinION and Illumina NextSeq 500 sequence data.