Data on preparation and characterization of an insect odorant receptor based biosensor

Roshan Khadka; Nihan Aydemir; Colm Carraher; Cyril Hamiaux; Damon Colbert; Jamal Cheema; Jenny Malmström; Andrew Kralicek; Jadranka Travas-Sejdic

doi:10.1016/j.dib.2018.11.018

Data on preparation and characterization of an insect odorant receptor based biosensor

Data Brief. 2018 Nov 7:21:2142-2148. doi: 10.1016/j.dib.2018.11.018. eCollection 2018 Dec.

Authors

Roshan Khadka^{1

2}, Nihan Aydemir^{1

2}, Colm Carraher³, Cyril Hamiaux³, Damon Colbert³, Jamal Cheema^{1

2}, Jenny Malmström^{2

4}, Andrew Kralicek³, Jadranka Travas-Sejdic^{1

2}

Affiliations

¹ Polymer Electronic Research Centre, School of Chemical Sciences, University of Auckland, Auckland 1023, New Zealand.
² MacDiarmid Institute for Advanced Materials and Nanotechnology, Wellington 6140, New Zealand.
³ The New Zealand Institute for Plant and Food Research Limited, Private Bag 92169, Auckland 1142, New Zealand.
⁴ Department of Chemical and Materials Engineering, University of Auckland, Auckland 1023, New Zealand.

Abstract

Insect Odorant receptors (OrXs) can be used as the recognition element in a biosensor as they demonstrate high levels of sensitivity and selectivity towards volatile organic compounds. Herein, we describe a method to express and purify insect odorant receptors and reconstitute them into artificial lipid bilayers (liposomes). These OrX/liposomes were covalently attached to a gold surface and characterized using quartz crystal microbalance with dissipation monitoring (QCM-D). The interaction of OrX/liposomes immobilized on a gold surface to positive and negative odorants were studied by means of electrochemical impedance spectroscopy (EIS) and QCM-D. The data presented in this article are related to the research article titled "An ultrasensitive electrochemical impedance-based biosensor using insect odorant receptors to detect odorants" [1].