Reduction of geomagnetic field (GMF) to near null magnetic field (NNMF) affects some Arabidopsis thaliana clock genes amplitude in a light independent manner

Abstract

Plant endogenous clock consists of self-sustained interlocked transcriptional/translational feedback loops whose oscillation regulates many circadian processes, including gene expression. Its free running rhythm can be entrained by external cues, which can influence all clock parameters. Among external cues, the geomagnetic field (GMF) has been demonstrated to influence plant growth and development. We evaluated the quantitative expression (qRT-PCR) of three clock genes (LHY, GI and PRR7) in time-course experiments under either continuous darkness (CD) or long days (LD) conditions in Arabidopsis thaliana seedlings exposed to GMF (∼40 μT) and Near Null Magnetic Field (NNMF; ∼40 nT) conditions. Under both LD and CD conditions, reduction of GMF to NNMF prompted a significant increase of the gene expression of LHY and PRR7, whereas an opposite trend was found for GI gene expression. Exposure of Arabidopsis to NNMF altered clock gene amplitude, regardless the presence of light, by reinforcing the morning loop. Our data are consistent with the existence of a plant magnetoreceptor that affects the Arabidopsis endogenous clock.

Keywords: Arabidopsis thaliana; Clock genes; Geomagnetic field; Internal clock; Near null magnetic field.