Genetic variants and haplotype combination in the bovine CRTC3 affected conformation traits in two Chinese native cattle breeds (Bos Taurus)

Sen Wu; Yue Ning; Sayed Haidar Abbas Raza; Chengtu Zhang; Le Zhang; Gong Cheng; Hongbao Wang; Nicola Schreurs; Linsen Zan

doi:10.1016/j.ygeno.2018.11.028

Genetic variants and haplotype combination in the bovine CRTC3 affected conformation traits in two Chinese native cattle breeds (Bos Taurus)

Genomics. 2019 Dec;111(6):1736-1744. doi: 10.1016/j.ygeno.2018.11.028. Epub 2018 Dec 5.

Authors

Sen Wu¹, Yue Ning², Sayed Haidar Abbas Raza², Chengtu Zhang³, Le Zhang², Gong Cheng⁴, Hongbao Wang⁴, Nicola Schreurs⁵, Linsen Zan⁶

Affiliations

¹ College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, PR China; Qinghai Academy of Animal Science and Veterinary Medicine, Qinghai University, Xining, Qinghai 810016, PR China.
² College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
³ Animal Husbandry and Veterinary Station in Xining City, Xining, Qinghai 810003, PR China.
⁴ College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, PR China; National Beef Cattle Improvement Center of Northwest A&F University, Yangling, Shaanxi 712100, PR China.
⁵ Animal Science, School of Agriculture and Environment, Massey University, Private Bag 11222, Palmerston North, New Zealand.
⁶ College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, PR China; National Beef Cattle Improvement Center of Northwest A&F University, Yangling, Shaanxi 712100, PR China. Electronic address: zanlinsen@163.com.

PMID: 30529539
DOI: 10.1016/j.ygeno.2018.11.028

Abstract

CREB-regulated transcription coactivator 3 (CRTC3) plays an extensive role in glucose and lipid metabolism. This study investigated the genetic variation and haplotype combination in CRTC3 and verified their contribution to bovine growth traits. Firstly, investigated the mRNA expression of CRTC3 in adult Qinchuan cattle and evaluated the effects that genetic variation of CRTC3 had on conformation and carcass traits in two Chinese cattle breeds (Qinchuan and Jiaxian). Four SNPs (single nucleotide polymorphisms) were identified including two in introns (SNP1: g.62652 A > G and SNP4: g.91297C > T) and two in exons (SNP2 g.62730C > T and SNP3: g.66478G > C). The association and haplotype combination results showed that there was an association with some growth and carcass traits(P < 0.05). Individuals with haplotype combination H1H1 (-AACCCCTT-) were associated with a conformation of a larger framed animal and an animal that produced a larger loin area. Variations in the CRTC3 genes and the haplotype combination H1H1 may be considered as molecular markers for carcass traits that are associated with more lean meat yield for use in cattle breeding programs in China.

Keywords: CRTC3; Conserved estimation; Growth traits; Molecular markers; SNP; mRNA expression.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Breeding*
Cattle / genetics*
China
Exons*
Haplotypes*
Polymorphism, Single Nucleotide*
Quantitative Trait, Heritable
Transcription Factors / genetics*

Substances

Transcription Factors