Suitability of dried DNA for long-range PCR amplification and HLA typing by next-generation sequencing

Hum Immunol. 2019 Feb;80(2):135-139. doi: 10.1016/j.humimm.2018.12.002. Epub 2018 Dec 4.

Abstract

Storage and stable shipment of genomic DNA are of great concern to laboratories that may need to perform testing off archived samples. There are some dry-state storage methods that are available that have the potential to provide a way to store samples at room temperature for long periods of time as well as offer a means to ship DNA to other facilities without the same safety concerns that come with shipping liquid samples. The recovered DNA should be of sufficient integrity such that downstream applications can be performed without concern of the sample quality. This work describes sample properties between two methods of DNA storage, dried (room temperature) and traditional (-80 °C). DNA was evaluated for purity, fragment length, and the ability to generate HLA typing using next-generation sequencing.

Keywords: DNA storage; Dry-state DNA; HLA; NGS.

MeSH terms

  • Cryopreservation
  • DNA / chemistry
  • DNA / genetics*
  • Dehydration
  • Feasibility Studies
  • HLA Antigens / genetics*
  • High-Throughput Nucleotide Sequencing / methods*
  • Histocompatibility Testing / methods*
  • Humans
  • Polymerase Chain Reaction / methods*
  • Specimen Handling

Substances

  • HLA Antigens
  • DNA