Effects of BPA on expression of apoptotic genes and migration of ovine trophectoderm (oTr1) cells during the peri-implantation period of pregnancy

Reprod Toxicol. 2019 Jan:83:73-79. doi: 10.1016/j.reprotox.2018.12.001. Epub 2018 Dec 5.

Abstract

Bisphenol A (BPA) is an endocrine-disrupting chemical used in the manufacture of many products used daily. In the present study, the effects of BPA (1 × 10-4 to 1 × 10-9 M) on migration and on the expression of some apoptotic genes were examined in vitro using ovine trophectoderm (oTr1) primary cell line. The results revealed that BPA at 1 × 10-9, 1 × 10-8 and 1 × 10-7M increased migration of oTr1 cells, while 1 × 10-6, 1 × 10-5 and 1 × 10-4 M BPA decreased cell migration. Regarding apoptosis, expression of the anti-apoptotic gene Bcl-2 mRNA was greater at 1 × 10-8 and 1 × 10-9 M BPA and was down-regulated at 1 × 10-4 to 1 × 10-7 M BPA; however, expression of pro-apoptotic genes (Bax, cathepsin B, caspase-3 and c-myc) was reduced at the higher concentrations of BPA. Results of this study suggest that BPA may impair implantation by decreasing migration of oTr1 cells and inhibiting apoptosis.

Keywords: Apoptosis; BPA; Migration; Pregnancy; Toxicity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis / drug effects
  • Apoptosis Regulatory Proteins / genetics
  • Benzhydryl Compounds / toxicity*
  • Cell Line
  • Cell Movement / drug effects
  • Embryo Implantation / drug effects*
  • Endocrine Disruptors / toxicity*
  • Female
  • Phenols / toxicity*
  • Pregnancy
  • Sheep

Substances

  • Apoptosis Regulatory Proteins
  • Benzhydryl Compounds
  • Endocrine Disruptors
  • Phenols
  • bisphenol A