Inhibitory effects of polysaccharide from Diaphragma juglandis fructus on α-amylase and α-d-glucosidase activity, streptozotocin-induced hyperglycemia model, advanced glycation end-products formation, and H2O2-induced oxidative damage

Qingran Meng; Feng Chen; Tiancun Xiao; Lianfu Zhang

doi:10.1016/j.ijbiomac.2018.12.011

Inhibitory effects of polysaccharide from Diaphragma juglandis fructus on α-amylase and α-d-glucosidase activity, streptozotocin-induced hyperglycemia model, advanced glycation end-products formation, and H₂O₂-induced oxidative damage

Int J Biol Macromol. 2019 Mar 1:124:1080-1089. doi: 10.1016/j.ijbiomac.2018.12.011. Epub 2018 Dec 3.

Authors

Qingran Meng¹, Feng Chen², Tiancun Xiao³, Lianfu Zhang⁴

Affiliations

¹ State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, PR China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, PR China; Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Beijing 100048, PR China; Department of Food, Nutrition and Packaging Sciences, Clemson University, Clemson, SC 29634, USA.
² Department of Food, Nutrition and Packaging Sciences, Clemson University, Clemson, SC 29634, USA.
³ Inorganic Chemistry Laboratory, University of Oxford, Oxford OX1 3QR, UK.
⁴ State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, PR China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, PR China; Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Beijing 100048, PR China; Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, Jiangnan University, Wuxi, Jiangsu 214122, PR China. Electronic address: lianfu@jiangnan.edu.cn.

PMID: 30521898
DOI: 10.1016/j.ijbiomac.2018.12.011

Abstract

In present study, the in vitro and in vivo hemolysis inhibitory, protective effect against reactive oxygen species (ROS) induced oxidative damage in L02 cells, hypoglycemic, and antiglycation activities of DJP-2, a pure polysaccharide fraction from Diaphragma juglandis fructus, were investigated. Results demonstrated that DJP-2 showed remarkable hemolysis inhibitory activity. Pretreatment with DJP-2 markedly weakened the oxidative damage induced by H₂O₂ in hepatic L02 cells via strengthening the cell viability. DJP-2 also showed clear in vivo and in vitro hypoglycemic activities. Besides, DJP-2 with the concentration of 3 mg/mL exerted more significant antiglycation activities than aminoguanidine during 30 days of incubation. The results obtained in this study would be beneficial for the application of DJP-2 to treat various diseases related to oxidative stress and AGEs. The elucidation of the potential bioactivities of DJP-2 will facilitate its further study and application in the functional food industry and pharmaceuticals industry.

Keywords: Antiglycation activity; Diaphragma juglandis fructus polysaccharide; Hemolysis inhibitory; Hypoglycemic.

MeSH terms

Animals
Cell Line
Glycation End Products, Advanced / metabolism*
Hydrogen Peroxide / metabolism*
Hyperglycemia / chemically induced
Hyperglycemia / drug therapy*
Hyperglycemia / metabolism
Hyperglycemia / pathology
Hypoglycemic Agents / chemistry
Hypoglycemic Agents / pharmacology*
Juglans / chemistry*
Mice
Oxidative Stress / drug effects*
Polysaccharides / chemistry
Polysaccharides / pharmacology*
Saccharomyces cerevisiae / enzymology*
Saccharomyces cerevisiae Proteins / antagonists & inhibitors*
Saccharomyces cerevisiae Proteins / chemistry
Streptozocin / toxicity
Sucrase / antagonists & inhibitors*
Sucrase / chemistry
alpha-Amylases / antagonists & inhibitors*
alpha-Amylases / chemistry

Substances

Glycation End Products, Advanced
Hypoglycemic Agents
Polysaccharides
Saccharomyces cerevisiae Proteins
Streptozocin
Hydrogen Peroxide
alpha-Amylases
Sucrase