Fluorescence polarization assay: Diagnostic evaluation for porcine brucellosis

Triveni Kalleshamurthy; Chaithra Yaranna; Ranjitha Shekar; Krithiga Natesan; Swati Sahay; Bibek Ranjan Shome; Habibur Rahman; Sukhadeo B Barbuddhe; Nagendra Nath Barman; Sailendra Kumar Das; Rajeswari Shome

doi:10.1016/j.mimet.2018.11.023

Fluorescence polarization assay: Diagnostic evaluation for porcine brucellosis

J Microbiol Methods. 2019 Jan:156:46-51. doi: 10.1016/j.mimet.2018.11.023. Epub 2018 Dec 3.

Affiliations

¹ Indian Council Agricultural Research-National Institute of Veterinary Epidemiology and Disease Informatics, Yelahanka, Bengaluru 560064, India; Jain University, Centre for Post Graduate Studies, Jaya Nagar, Bengaluru- 560041, India.
² Indian Council Agricultural Research-National Institute of Veterinary Epidemiology and Disease Informatics, Yelahanka, Bengaluru 560064, India.
³ International Livestock Research Institute, NASC Complex, CG Centre, Pusa, New Delhi 110 012, India.
⁴ ICAR-National Research Centre on Meat, Chengicherla, Hyderabad 500092, India.
⁵ Department of Microbiology, College of Veterinary Science, Khanapara, Guwahati 781022, India.
⁶ Indian Council Agricultural Research-National Institute of Veterinary Epidemiology and Disease Informatics, Yelahanka, Bengaluru 560064, India. Electronic address: rajeswarishome@gmail.com.

PMID: 30521866
DOI: 10.1016/j.mimet.2018.11.023

Abstract

Brucellosis in pigs, caused by the bacterium Brucella suis, is an important zoonotic infection. In the present study, fluorescence polarization assay (FPA) was standardized and compared with indirect enzyme linked immunosorbent assay (iELISA) and competitive ELISA (cELISA) for diagnosis of porcine brucellosis. Test performances were evaluated using representative panel (n = 100), samples from swine brucellosis outbreak (n = 300), samples from brucellosis suspected animals (n = 291) and sera samples from apparently healthy animals (n = 1121). With panel samples, the FPA cut-off ≥11ΔmP was arrived with sensitivity (Se) and specificity (Sp) of 95.00 and 98.75%, respectively. Testing of samples from swine brucellosis outbreak, the diagnostic Se and Sp of 100 and 95.14% by iELISA, 73.91 and 100% by cELISA and 86.96 and 100% by FPA, respectively were recorded. Similarly, in case of swine brucellosis suspected samples, relative performance of FPA with cELISA had revealed higher kappa value of 0.864 with an accuracy of 93.47. Indirect ELISA was found to be highly sensitive but showed cross reactivity mainly for Yersinia enterocolitica O9 antibodies than cELISA and FPA. The high specificity of FPA test recorded in various types of samples in the study indicated that, FPA could serve as confirmatory test for individual animal diagnosis, outbreak confirmation, surveillance and quarantine of swine brucellosis cases.

Keywords: ELISA; FPA; RBPT; Seroprevalence; Swine.

Publication types

Comparative Study
Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antibodies, Bacterial / blood*
Brucella suis / isolation & purification*
Brucellosis / veterinary*
Cross Reactions
Enzyme-Linked Immunosorbent Assay
Fluorescence Polarization Immunoassay / methods*
Sensitivity and Specificity
Swine
Swine Diseases / diagnosis*

Substances

Antibodies, Bacterial