In Denmark, 45% of all incident cervical cancers are detected in underscreened women. High-level evidence supports, that non-participants in cervical cancer screening are better reached by HPV self-sampling than by regular physician-based screening, including increased uptake among un- or underscreened women. A high compliance to follow-up among HPV-positive self-samplers has been reported. In the new Danish cervical cancer screening guidelines, the regions are endorsed to offer HPV self-sampling to women, who are due to receive the second screening reminder, and in this manner potentially improve cervical cancer prevention.