In this paper, we separately fabricated the poly ( ε -caprolactone) (PCL) scaffolds containing epidermal growth factor (EGF) by using our-self fabricated electrospinning machine for tissue regeneration application. Several fundamental properties, including the dimensions, wettability, and EGF release profiles, of the fabricated EGF-PCL bead, fibrous, and multistructured scaffolds were characterized by using the scanning electron microscopy (SEM), contact angle goniometer, and vertical diffusion system. The EGF release profiles of three scaffolds were measured for 200 h, and the multistructured scaffold performed stable and long EGF release properties. Furthermore, the in vitro and in vivo experiments were conducted to evaluate the performance of three types of EGF-PCL scaffolds. The NIH-3T3 fibroblast cells were cultured on the scaffolds to study the effects of the released EGF by using the Alamar Blue Cell Viability Assays, fluorescence image analysis, and SEM. The animal experiment demonstrated that the multistructured EGF-PCL scaffold achieved 95% of the wound healing rate to the cut-damaged wounds after 16 days. Moreover, the histopathological skin tissue showed that the wound regenerated well after the treatment of the multistructured EGF-PCL scaffold. The developed multistructured EGF-PCL scaffold has a high potential for the wound healing applications.