Broadband homodecoupled time-shared 1H-13C and 1H-15N HSQC experiments

Pau Nolis; Kumar Motiram-Corral; Míriam Pérez-Trujillo; Teodor Parella

doi:10.1016/j.jmr.2018.11.005

Broadband homodecoupled time-shared ¹H-¹³C and ¹H-¹⁵N HSQC experiments

J Magn Reson. 2019 Jan:298:23-30. doi: 10.1016/j.jmr.2018.11.005. Epub 2018 Nov 22.

Authors

Pau Nolis¹, Kumar Motiram-Corral¹, Míriam Pérez-Trujillo¹, Teodor Parella²

Affiliations

¹ Servei de Ressonància Magnètica Nuclear, Universitat Autònoma de Barcelona, E-08193, Bellaterra, Barcelona, Catalonia, Spain.
² Servei de Ressonància Magnètica Nuclear, Universitat Autònoma de Barcelona, E-08193, Bellaterra, Barcelona, Catalonia, Spain. Electronic address: teodor.parella@uab.cat.

PMID: 30502625
DOI: 10.1016/j.jmr.2018.11.005

Abstract

The concepts of pure-shift NMR and time-shared NMR are merged in a single experiment. A ¹³C/¹⁵N time-shared version of the real-time BIRD-based broadband homodecoupled HSQC experiment is described. This time-efficient approach affords simultaneously ¹H-¹³C and ¹H-¹⁵N pure-shift HSQC spectra in a single acquisition, while achieving substantial gains in both sensitivity and spectral resolution. We also present a related ¹³C/¹⁵N-F2-coupled homodecoupled version of the CLIP-HSQC experiment for the simultaneous measurement of ¹J_CH and ¹J_NH from the simplified doublets observed along the direct dimension. Finally, a novel J-resolved HSQC experiment has been designed for the simple and automated determination of both ¹J_CH/¹J_NH from a 2D J-resolved spectrum.

Keywords: HSQC; J-resolved HSQC; NMR; Proton-carbon coupling constants; Proton-nitrogen coupling constants; Pure-shift HSQC; Time-shared NMR.

Publication types

Research Support, Non-U.S. Gov't