Microchip Electrophoresis Containing Electrodes for Integrated Electrochemical Detection

Lucas Paines Bressan; Dosil Pereira de Jesus; Dulan Bandara Gunasekara; Susan Marie Lunte; José Alberto Fracassi da Silva

doi:10.1007/978-1-4939-8964-5_5

Microchip Electrophoresis Containing Electrodes for Integrated Electrochemical Detection

Methods Mol Biol. 2019:1906:79-85. doi: 10.1007/978-1-4939-8964-5_5.

Authors

Lucas Paines Bressan¹, Dosil Pereira de Jesus¹, Dulan Bandara Gunasekara², Susan Marie Lunte³, José Alberto Fracassi da Silva⁴

Affiliations

¹ Chemistry Institute, State University of Campinas, Campinas, SP, Brazil.
² Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
³ Ralph N. Adams Institute for Bioanalytical Chemistry, University of Kansas, Lawrence, KS, USA.
⁴ Chemistry Institute, State University of Campinas, Campinas, SP, Brazil. fracassi@iqm.unicamp.br.

PMID: 30488386
DOI: 10.1007/978-1-4939-8964-5_5

Abstract

Microchip electrophoresis is a versatile separation technique. Electrochemical detection is suitable to apply to microdevices due to its easy integration to the fabrication process and good sensitivity and selectivity. Here we describe the procedures to prepare Pt band electrodes deposited on glass to couple to polydimethylsiloxane (PDMS) microchips aiming the separation and detection of nitrite using an isolated potentiostat.

Keywords: Amperometric detection; Gated injection; Isolated potentiostat; Polydimethylsiloxane; Reactive nitrogen species.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Biosensing Techniques / instrumentation
Dimethylpolysiloxanes / chemistry*
Electrodes
Electrophoresis, Microchip / instrumentation*
Electrophoresis, Microchip / methods
Glass
Nitrites / analysis*

Substances

Dimethylpolysiloxanes
Nitrites
baysilon