Antioxidant Properties of Glial Cell-Derived Neurotrophic Factor (GDNF)

T A Mishchenko; E V Mitroshina; T V Shishkina; M B Vedunova

doi:10.1007/s10517-018-4335-z

Antioxidant Properties of Glial Cell-Derived Neurotrophic Factor (GDNF)

Bull Exp Biol Med. 2018 Dec;166(2):293-296. doi: 10.1007/s10517-018-4335-z. Epub 2018 Nov 28.

Authors

T A Mishchenko¹, E V Mitroshina¹, T V Shishkina¹, M B Vedunova²

Affiliations

¹ N. I. Lobachevsky Nizhny Novgorod National Research State University, Nizhny Novgorod, Russia.
² N. I. Lobachevsky Nizhny Novgorod National Research State University, Nizhny Novgorod, Russia. mvedunova@yandex.ru.

PMID: 30488198
DOI: 10.1007/s10517-018-4335-z

Abstract

We propose an in vitro model of chronic oxidative stress based on the use of glucose oxidase. Oxidative stress modeling leads to a significant increase in the number of dead cells in culture. It was shown that the glial cell-derived neurotrophic factor exhibits a pronounced anti-oxidant effect. Preventive application of 1 ng/ml glial cell-derived neurotrophic factor significantly reduced the percentage of dead cells in culture.

Keywords: chronic oxidative stress; glial cell-derived neurotrophic factor; neuroprotection; primary dissociated cultures.

MeSH terms

Animals
Antioxidants / pharmacology*
Apoptosis / drug effects
Cell Survival / drug effects
Embryo, Mammalian
Glial Cell Line-Derived Neurotrophic Factor / pharmacology*
Glucose Oxidase / antagonists & inhibitors*
Glucose Oxidase / pharmacology
Hippocampus / cytology
Hippocampus / drug effects*
Hippocampus / metabolism
Mice
Mice, Inbred C57BL
Neurons / cytology
Neurons / drug effects*
Neurons / metabolism
Oxidative Stress
Primary Cell Culture

Substances

Antioxidants
Gdnf protein, mouse
Glial Cell Line-Derived Neurotrophic Factor
Glucose Oxidase