Exopolysaccharide from Pantoea sp. BCCS 001 GH isolated from nectarine fruit: production in submerged culture and preliminary physicochemical characterizations

Seyyed Vahid Niknezhad; Mohammad Hossein Morowvat; Ghasem Najafpour Darzi; Aida Iraji; Younes Ghasemi

doi:10.1007/s10068-018-0409-y

Exopolysaccharide from Pantoea sp. BCCS 001 GH isolated from nectarine fruit: production in submerged culture and preliminary physicochemical characterizations

Food Sci Biotechnol. 2018 Jun 12;27(6):1735-1746. doi: 10.1007/s10068-018-0409-y. eCollection 2018 Dec.

Authors

Seyyed Vahid Niknezhad^{1

2}, Mohammad Hossein Morowvat^{2

3}, Ghasem Najafpour Darzi¹, Aida Iraji⁴, Younes Ghasemi^{2

3}

Affiliations

¹ 1Department of Chemical Engineering, Faculty of Engineering, Noshirvani University of Technology, Babol, Iran.
² 2Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, P.O. Box 71345-1583, Shiraz, Iran.
³ 3Department of Pharmaceutical Biotechnology, School of Pharmacy, Shiraz University of Medical Sciences, P.O. Box 71345-1583, Shiraz, Iran.
⁴ 4Central Research Laboratory, Shiraz University of Medical Science, Shiraz, Iran.

Abstract

Exopolysaccharide (EPS), as potential microbial base polysaccharide source, has plenty of applications due to its unique physicochemical structure. A Pantoea sp. BCCS 001 GH bacterium with the ability to produce a high amount of EPS was identified by 16S rRNA gene sequencing and biochemical tests. The synthesis of EPS by Pantoea sp. BCCS 001 GH was 13.50 g/L in 48 h when sucrose was used as substrate. The proposed protocol was desirably rapid for massive prodcution of EPS and showed the remarkable impact of sucrose and disodium hydrogen phosphate, peptone, Triton x-100 and 2% (v/v) inoculum size on the yields of EPS production. The EPS was mainly composed of glucose and galactose in a relative molar ration (glucose/galactose) of 85.18:14.82, respectively. The preliminary characterization showed the average molecular-weight of EPS is about 2.522 × 10⁶ Da. The microscopics morphology of polymer was formed irregularly shaped structures.

Keywords: Exopolysaccharide; Pantoea sp. BCCS 001 GH; Physicochemical structure.