The effect of the low temperature (4 ºC) on the orga-nization of actin filaments (microfilaments) of cells of different growth zones of the root of Arabidopsis thaliana (L.) have been studied. It was found that cold treatment inhibits growth of the main root and gives its morphology, causing a large number of deformed (ectopic) root hairs in the zone of differentiation. The temporal relationship of the disorientation and the organization of actin filaments and the detected changes of growth and morphology of roots under conditions of cold factor is shown. It has been found that the most sensitive to the cold are actin filaments of root hairs, meristematic cells, cells of elongation zone, and all epidermal cells of the root zones of A. thaliana.