Comparative Genomics of Serial Candida glabrata Isolates and the Rapid Acquisition of Echinocandin Resistance during Therapy

Antimicrob Agents Chemother. 2019 Jan 29;63(2):e01628-18. doi: 10.1128/AAC.01628-18. Print 2019 Feb.

Abstract

The opportunistic pathogen Candida glabrata shows a concerning increase in drug resistance. Here, we present the analysis of two serial bloodstream isolates, obtained 12 days apart. Both isolates show pan-azole resistance and echinocandin resistance was acquired during the sampling interval. Genome sequencing identified nine nonsynonymous SNVs between the strains, including a S663P substitution in FKS2 and previously undescribed SNVs in MDE1 and FPR1, offering insight into how C. glabrata acquires drug resistance and adapts to a human host.

Keywords: Candida; Candida glabrata; candidiasis; comparative genomics; fungus; resistance.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antifungal Agents / pharmacology
  • Candida glabrata / drug effects*
  • Candida glabrata / genetics*
  • Candidiasis / microbiology
  • Echinocandins / pharmacology*
  • Fungal Proteins / genetics
  • Genomics / methods*
  • Humans
  • Microbial Sensitivity Tests

Substances

  • Antifungal Agents
  • Echinocandins
  • Fungal Proteins