In this study, a mixture of carbamates (CRBs) degrading Carb.1b strain was isolated from soil. Based on the morphology and 18S rRNA sequence analysis, the strain was identified as an Ascochyta sp. CBS 237.37 with accession number MG786925. The isolate was employed in two growth mediums (added carbon and carbon-free) enriched with varied concentrations of CRBs ranging from 25 to 85 mg L-1 to assess its degradation efficacy. As determined by the Response Surface Methodology (RSM), optimum parameters for the degradation were: pH value of 7.5 and temperature of 28 °C. The degradation was inhibited at higher concentrations and was found to be 91.2%/94.8%, 67.25%/71.75%, 55.81%/59.81%, 46.85%/49.57% and 36%/40.80% (in carbon-free/added carbon) after 20 d. The removal of the higher concentration CRBs was comparatively slower, and the obtained degradation rate constant (Kavg) 0.03412 d-1. Added carbon and carbon-free medium removed over 86.7%/90.15% of CRBs (85 mgL-1) with the half-life (t1/2) of 26 d and R2 ranging from 0.982 to 0.999; indicating the high tolerance of carb.1b strain towards CRBs. Residual analysis of CRBs biodegradation was performed using GC/MS analysis. This is the first report of degradation of a mixture of CRBs by Ascochyta sp. CBS 237.37. The results of this study can possibly impact the development strategies of bioremediation for the elimination of CRBs.
Keywords: Ascochyta; Carbamate; Fungi; In-situ Bioremediation; Mycoremediation.
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