Single-cell 3D electro-rotation

Liang Huang; Peng Zhao; Fei Liang; Wenhui Wang

doi:10.1016/bs.mcb.2018.06.013

Single-cell 3D electro-rotation

Methods Cell Biol. 2018:148:97-116. doi: 10.1016/bs.mcb.2018.06.013. Epub 2018 Jul 20.

Authors

Liang Huang¹, Peng Zhao¹, Fei Liang¹, Wenhui Wang²

Affiliations

¹ State Key Laboratory of Precision Measurement Technology and Instrument, Department of Precision Instruments, Tsinghua University, Beijing, China.
² State Key Laboratory of Precision Measurement Technology and Instrument, Department of Precision Instruments, Tsinghua University, Beijing, China. Electronic address: wwh@tsinghua.edu.cn.

PMID: 30473076
DOI: 10.1016/bs.mcb.2018.06.013

Abstract

Single-cell rotation is a fundamental manipulation used in a wide range of biotechnological applications such as cell injection and enucleation. However, there are currently few methods for the 3D rotation of single cells. Here, this chapter presents different biochip platforms based on a dielectrophoresis technique to achieve 3D rotation. In-plane (yaw) and out-of-plane rotation (pitch) can be achieved by applying different AC signal configurations, respectively. This use of 3D rotation facilitates several applications. For example, in-plane rotation can be used to measure the rotation spectra, and this can be used to estimate the dielectric parameters. The out-of-plane rotation can help reconstruct 3D cell models.

Keywords: 3-Dimension electrodes; 3-Dimension rotation; 3D electrodes; 3D rotation; DEP; Dielectrophoresis; Microfluidics; Single cell.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Electrodes
Microfluidics / methods*
Rotation
Single-Cell Analysis / methods*