A Moso Bamboo Drought-Induced 19 Protein, PeDi19-4, Enhanced Drought and Salt Tolerance in Plants via the ABA-Dependent Signaling Pathway

Min Wu; Ronghao Cai; Huanlong Liu; Fei Li; Yang Zhao; Yan Xiang

doi:10.1093/pcp/pcy196

A Moso Bamboo Drought-Induced 19 Protein, PeDi19-4, Enhanced Drought and Salt Tolerance in Plants via the ABA-Dependent Signaling Pathway

Plant Cell Physiol. 2019 Jul 1;60(7):e1-e14. doi: 10.1093/pcp/pcy196.

Authors

Min Wu¹, Ronghao Cai¹, Huanlong Liu¹, Fei Li², Yang Zhao¹, Yan Xiang^{1

2}

Affiliations

¹ National Engineering Laboratory of Crop Stress Resistance Breeding, Anhui Agricultural University, Hefei 230036, China.
² Laboratory of Modern Biotechnology, School of Forestry and Landscape Architecture, Anhui Agricultural University, Hefei 230036, China.

PMID: 30452736
DOI: 10.1093/pcp/pcy196

Abstract

Here, 10 drought-induced 19 (Di19) proteins from Phyllostachys edulis were analyzed and an important stress-related candidate gene (PeDi19-4) was isolated based on analysis of phylogenetic relationships and expression profiles. PeDi19-4 is a nuclear localization protein that can bind the conserved TACA(A/G)T sequence, as determined using enzyme-linked immunosorbent assay (EMSA). PeDi19-4 has no transcriptional activity in yeast but functions as a transcription activator in plants. Overexpression of PeDi19-4 in rice and Arabidopsis thaliana enhanced drought and salt tolerance as determined through phenotypic analysis and the use of stress-associated physiological indicators. PeDi19-4 transgenic plants showed increased sensitivity to ABA during seed germination and early seedling growth. Additionally, transgenic rice accumulated more ABA than wild-type plants under drought and salt stress conditions. Moreover, the stomata of PeDi19-4-overexpressing plants changed significantly with ABA treatment. RNA sequencing revealed that PeDi19-4 regulated the expression of a wide spectrum of stress-/ABA-responsive differentially expressed genes. The stress-responsive genes (OsZFP252 and OsNAC6) and ABA-responsive genes (OsBZ8 and OsbZIP23) were direct targets of PeDi19-4. Our research indicated that PeDi19-4 enhanced drought and salt tolerance in plants via the ABA-dependent signaling pathway.

Keywords: ABA; Drought and salt stresses; Moso bamboo; PeDi19-4; Target genes; Transcription activator.

Publication types

Retracted Publication