Sustainable biosynthesis of curdlan from orange waste by using Alcaligenes faecalis: A systematically modeled approach

Ali Mohsin; Jingyun Sun; Imran Mahmood Khan; Haifeng Hang; Muhammad Tariq; Xiwei Tian; Waqas Ahmed; Sobia Niazi; Yingping Zhuang; Ju Chu; Muhammad Zubair Mohsin; Salim-Ur-Rehman; Meijin Guo

doi:10.1016/j.carbpol.2018.10.047

Sustainable biosynthesis of curdlan from orange waste by using Alcaligenes faecalis: A systematically modeled approach

Carbohydr Polym. 2019 Feb 1:205:626-635. doi: 10.1016/j.carbpol.2018.10.047. Epub 2018 Oct 25.

Authors

Affiliations

¹ State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, PR China.
² State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, PR China.
³ State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, PR China. Electronic address: hanghaifeng@ecust.edu.cn.
⁴ State Key Laboratory of Environmental Engineering, East China University of Science and Technology, Shanghai 200237, PR China.
⁵ Center of Agriculture Biochemistry and Biotechnology, University of Agriculture, Faisalabad 38040, Pakistan.
⁶ National Institute of Food Science and Technology, University of Agriculture, Faisalabad 38040, Pakistan.
⁷ State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, PR China. Electronic address: guo_mj@ecust.edu.cn.

PMID: 30446148
DOI: 10.1016/j.carbpol.2018.10.047

Abstract

This study presents an engineered approach for sustainable biosynthesis of curdlan by Alcaligenes faecalis using orange peels. To confirm the substrate suitability a four step study was organized. Firstly, drying of substrate was carried within temperature range of 60-120 °C, along with the application of moisture diffusion control model. Secondly, fermentation medium was obtained via saccharification and detoxification, releasing highest sugar at 72.34 g/L with phenolics removal of 95-98%. Thirdly, curdlan fermentation was conducted in detoxified orange peel hydrolysate followed by optimization of batch culture fermentation via kinetic modeling using Logistic and Luedeking-Piret equations. In 5 L bioreactor, highest specific growth rate (μ_m = 0.233/h), highest curdlan production (P_m = 23.24 g/L) and growth associated rate constant (α = 3.403) were achieved. Moreover, the total sugar consumption and conversion rates were 83.27% and 53.20%. Lastly, characterization techniques such as FTIR, NMR, XRD, TGA, HPGPC and EDS were applied to biosynthesized curdlan for qualitative validation.

Keywords: Batch culture fermentation; Curdlan; Kinetics; Orange peels; Sustainable biosynthesis.