A hybridization-triggered DNAzyme cascade assay for enzyme-free amplified fluorescence detection of nucleic acids

Analyst. 2018 Dec 17;144(1):143-147. doi: 10.1039/c8an01796d.

Abstract

An enzyme-free and ultrasensitive fluorescence assay for the detection of nucleic acids was successfully established by a hybridization-triggered DNAzyme cascade (HTDC). This simple and cost-effective sensor has good sensitivity, selectivity and the capability for detection of target DNA from complex fluids.

MeSH terms

  • Base Sequence
  • Biological Assay / methods*
  • DNA / analysis*
  • DNA / chemistry
  • DNA, Catalytic / chemistry*
  • Fluorescence
  • Fluorescent Dyes / chemistry
  • Nucleic Acid Amplification Techniques / methods*
  • Nucleic Acid Hybridization
  • Zinc / chemistry

Substances

  • DNA, Catalytic
  • Fluorescent Dyes
  • DNA
  • Zinc