Pseudo-Continuous Flow FTIR System for Glucose, Fructose and Sucrose Identification in Mid-IR Range

Micromachines (Basel). 2018 Oct 13;9(10):517. doi: 10.3390/mi9100517.

Abstract

In this paper, we present a new FTIR-based microfluidic system for Glucose, Fructose and Sucrose detection. The proposed microfluidic system is based on a pseudo-continuous flow coupled to a microscope-FTIR instrument. The detection and characterization of sugar samples were performed by recording their absorption spectrum in the wavelength range 700⁻1000 cm - 1 of the Mid-IR region. The proposed pseudo-continuous flow system is designed to improve the uniformity of the sample distribution in the analyzed area versus conventional systems. The obtained results for different sugars concentrations, show a very low measurement error of 4.35% in the absorption peak intensity, which is ten times lower than the error obtained using the conventional measurements.

Keywords: FTIR spectroscopy; Fructose; Glucose; Sucrose; absorption spectrum; microscopy-FTIR spectrometer; mid-IR absorption spectroscopy; pseudo-continuous flow; sugars detection and quantification.