Isolating dissolved organic matter (DOM) is a preliminary step that improves the accuracy of its characterization. In this study, DOM in brackish water was clearly separated and evaluated by multiple characterization analyses. The sample was divided into three fractions by preparative high-performance liquid chromatography (preparative HPLC) according to molecular size. The homogeneity of each fraction was estimated by analytical size exclusion chromatography (SEC) and fluorescence excitation-emission matrix (FEEM). Pyrolysis gas chromatography-mass spectrometry (Py-GC/MS) and liquid chromatography-organic carbon detection (LC-OCD) were used to characterize the physicochemical properties of each fraction. Py-GC/MS revealed that Fraction 1 consisted of evenly distributed organic matter in order polysaccharides, proteins, polyhydroxy aromatics, lignins, and lipids. However, Fraction 2 was primarily composed of dominant lipids and low portion of proteins, and Fraction 3 was composed predominantly of lignins and lipids. The LC-OCD results showed that Fractions 1 and 2 had similar organic carbon (OC) compositions: a humic substance (ca. 37%), building blocks (ca. 10%), and neutrals (ca. 37%), whereas Fraction 3 contained a high proportion of neutrals (62%). In the fouling experiments, the distinct DOM characteristics in each fraction resulted in different declining flux behaviors, ranked as: Fraction 2 > Fraction 1 > Fraction 3.
Keywords: Brackish water fractionation; Fouling potential; Organic matter characterization; Preparative high-performance liquid chromatography.
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