Unobtrusive monitoring of vital signs is relevant for both medical (patient monitoring) and non-medical applications (e.g., stress and fatigue monitoring). In this paper, we focus on the use of imaging photoplethysmography (iPPG). High frame rate videos were acquired by using a monochrome camera and an optical band-pass filter ([Formula: see text] nm). To enhance iPPG signal, we investigated the use of independent component analysis (ICA) pre-processing applied to iPPG signal from different regions of the face. Methodology was tested on [Formula: see text] healthy volunteers. Heart rate (HR) and standard time and frequency domain descriptors of heart rate variability (HRV), simultaneously extracted from videos and ECG data, were compared. A mean absolute error (MAE) about 3.812 ms was observed for normal-to-normal intervals with or without ICA pre-processing. Smaller MAE values of frequency domain descriptors were observed when ICA pre-processing was used. The impact of both video frame rate and video signal interval were also analyzed. All the results support the conclusion that proposed ICA pre-processing can effectively improve the HR and HRV assessment from iPPG.