Interleukin-1β influences functional regeneration following nerve injury in mice through nuclear factor-κB signaling pathway

Abstract

This study focuses on investigating the role of interleukin-1β (IL-1β) in functional regeneration following nerve injury in mice. A microarray-based mRNA profiling study was used to analyze the expression level of IL-1β in peripheral nerve regeneration. Quantitative real-time polymerase chain reaction and Western blot were applied to assess the IL-1β expressions of C57BL/6J-crush and C57BL/6J-crush+IL-1β mice at different post-injury time-points after the standard sciatic nerve crush injury. The outcomes of nerve regeneration were evaluated by behavioral tests. IL-1β was found to be up-regulated in peripheral nerve regeneration and significantly raised on the 3rd day and returned to normal levels on the 14th day after nerve injury. Compared with C57BL/6J-crush+IL-1β mice, the nerve regeneration of C57BL/6J-crush mice was worse after nerve crush injury. IL-1β increased mechanical sensitivity and stimulated amplitude. IL-1β could benefit the recovery of sciatic nerve crush injury by facilitating nerve regeneration.

Keywords: functional recovery; interleukin-1β; nerve crush injury; nerve regeneration.